Dettaglio ricerca - Difesa e Certificazione

Formato application/pdf; version=1.7

Modificato 2023-10-31T14:35:15Z

Creatore Alessandro Infantino, Cosimo Taiti, Alessandro Grottoli, Stefano Mancuso, Corrado Costa and Stefania Garzoli

Creato 2023-10-31T14:28:32Z

Oggetto chemical volatile composition; diagnosis; fungi; volatome

Titolo Examination of Volatile Signatures of Fusarium Bulb Rot in Garlic Using Proton-Transfer-Reaction Time-of-Flight Mass Spectrometry and Solid-Phase Microextraction Gas Chromatography/Mass Spectrometry

Tipo Contenuto application/pdf

Ultima Modifica 2023-10-31T14:35:15Z

Parole chiavi chemical volatile composition; diagnosis; fungi; volatome

Autore Alessandro Infantino, Cosimo Taiti, Alessandro Grottoli, Stefano Mancuso, Corrado Costa and Stefania Garzoli

Data di creazione 2023-10-31T14:28:32Z

Salva data 2023-10-31T14:35:15Z

metadata.OfficeOpenXMLCore.SUBJECT Fusarium bulb rot, caused by Fusarium proliferatum, is a worldwide disease of garlic, both in the open field and during storage. Early diagnosis of the disease during storage is difficult due to the morphology of the bulbs and cloves. Volatile organic compounds (VOCs) are secondary metabolites produced by several microorganisms, including phytopathogenic fungi and bacteria. In recent years, the development of several techniques for the detection and characterization of VOCs has prompted their use, among others, as a diagnostic tool for the early and non-destructive analysis of many diseases of species of agricultural interest. In this paper, proton-transfer-reaction time-of-flight mass spectrometry (PTR-ToF-MS) and solid-phase microextraction gas chromatography/mass spectrometry (SPME-GC/MS) were successfully utilized to characterize the volatolome of commercial garlic cloves, artificially and naturally infected with F. proliferatum, for the early discrimination between diseased and healthy ones. A partial least squares discriminant analysis (PLSDA) and a principal component analysis (PCA) allowed for the separation of infected and healthy cloves and the identification of specific VOCs produced by the fungus during the infection. The results obtained in this work could be utilized for the development of simpler, more economical, and more portable devices for the early detection of infected garlic bulbs during storage.