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Infantino et al_VOCs garlic.pdf
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- 15/11/23 8.46 di Andrea Gentili
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- 15/11/23 8.46 di Andrea Gentili
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application/pdf; version=1.7
2023-10-31T14:35:15Z
Alessandro Infantino, Cosimo Taiti, Alessandro Grottoli, Stefano Mancuso, Corrado Costa and Stefania Garzoli
2023-10-31T14:28:32Z
chemical volatile composition; diagnosis; fungi; volatome
Examination of Volatile Signatures of Fusarium Bulb Rot in Garlic Using Proton-Transfer-Reaction Time-of-Flight Mass Spectrometry and Solid-Phase Microextraction Gas Chromatography/Mass Spectrometry
application/pdf
2023-10-31T14:35:15Z
chemical volatile composition; diagnosis; fungi; volatome
Alessandro Infantino, Cosimo Taiti, Alessandro Grottoli, Stefano Mancuso, Corrado Costa and Stefania Garzoli
2023-10-31T14:28:32Z
2023-10-31T14:35:15Z
Fusarium bulb rot, caused by Fusarium proliferatum, is a worldwide disease of garlic, both in the open field and during storage. Early diagnosis of the disease during storage is difficult due to the morphology of the bulbs and cloves. Volatile organic compounds (VOCs) are secondary metabolites produced by several microorganisms, including phytopathogenic fungi and bacteria. In recent years, the development of several techniques for the detection and characterization of VOCs has prompted their use, among others, as a diagnostic tool for the early and non-destructive analysis of many diseases of species of agricultural interest. In this paper, proton-transfer-reaction time-of-flight mass spectrometry (PTR-ToF-MS) and solid-phase microextraction gas chromatography/mass spectrometry (SPME-GC/MS) were successfully utilized to characterize the volatolome of commercial garlic cloves, artificially and naturally infected with F. proliferatum, for the early discrimination between diseased and healthy ones. A partial least squares discriminant analysis (PLSDA) and a principal component analysis (PCA) allowed for the separation of infected and healthy cloves and the identification of specific VOCs produced by the fungus during the infection. The results obtained in this work could be utilized for the development of simpler, more economical, and more portable devices for the early detection of infected garlic bulbs during storage.
- Versione 1.0Da Andrea Gentili. su 15/11/23 8.46
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